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. 2016 Jan 11;47:16. doi: 10.1186/s13567-015-0294-9

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© Xie et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Immuno-EM of different kinds of VLP. RBS, NHV, 220HV, and CHV were immune-labeled with gold particles using mouse anti-his antibodies (A) or mouse anti-VLP sera (B) as the first antibody as indicated. The black dots are the 5 nm gold particles conjugated with goat anti-mouse secondary antibodies and the bars indicate 100 nm.