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. 2016 Jan 12;7:5. doi: 10.1186/s13293-015-0056-4

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© Johansson et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Padlock hybridization with ACTB, PCDH11X/Y and NLGN4X/Y. a, b. Male MO sections simultaneously hybridized with one padlock probe for ACTB, five probes for PCDH11X and five for PCDH11Y. a, b The same region of the section with staining for nuclei and ACTB (a) and PCDH11X (cyan) and PCDH11Y (red) (b). Most stained cells express either PCDH11X or PCDH11Y. Rarely, some cells express both X and Y transcripts (marked with arrows). c, d Male MO section simultaneously hybridized with one padlock probe for ACTB, four probes for NLGN4X and four for NLGN4Y. Nuclei and ACTB staining are shown in c, and NLGN4X (cyan) and NLGN4Y (red) staining in d. e Male SC section simultaneously hybridized with five probes for PCDH11X (cyan), five for PCDH11Y (blue), four probes for NLGN4X (red) and four for NLGN4Y (purple)