Table 2.

Distribution of cells with NLGN4X/Y transcripts: amount of cells with NLGN4X/Y signals

Sample	Tissue	Gene	Gender	Cells without signals	Cells with X = 1	Cells with Y = 1	Cells with X + Y > = 2	Cells with X > 1 Y = 0	Cells with Y > 1 X = 0	Total cell counts
E27_S48	SC	NLGN4	Female	3098	364	3	4	45	0	3514
E31_S56	SC	NLGN4	Female	2955	493	3	0	94	1	3546
E49_S81	SC	NLGN4	Female	4225	350	4	0	47	0	4626
E49_S82	SC	NLGN4	Female	4767	297	9	1	23	0	5097
E33_S62	MO	NLGN4	Female	12,552	1917	20	4	284	0	14,777
E34_S61	MO	NLGN4	Female	22,028	3586	40	25	674	5	26,358
E33_S63	MO	NLGN4	Female	15,497	1652	49	14	177	1	17,390
E34_S63	MO	NLGN4	Female	24,119	2871	76	33	381	5	27,485
E26_S65	SC	NLGN4	Male	3165	200	303	36	26	25	3755
E32_S29	SC	NLGN4	Male	3917	319	447	75	25	36	4819
E32_S84	SC	NLGN4	Male	4120	310	381	69	28	31	4939
E61_S39	MO	NLGN4	Male	5283	379	414	58	19	47	6200
E61_S89	MO	NLGN4	Male	6123	633	710	117	56	75	7714
E45_S48	MO	NLGN4	Male	16,555	1564	1580	295	133	144	20,271
E41_S58	MO	NLGN4	Male	5719	563	584	421	50	49	7386
E45_S50	MO	NLGN4	Male	16,356	1644	1261	391	155	75	19,882
E58_S82	MO	NLGN4	Male	8219	1205	1102	491	200	114	11,331
E61_S72	MO	NLGN4	Male	6405	273	645	68	19	62	7472
E45_S329	Midbrain	NLGN4	Male	24,917	1623	2226	292	107	153	29,318
E47_S77	Midbrain	NLGN4	Male	5774	558	772	165	50	67	7386
E45_S329	Midbrain	NLGN4	Male	21,915	1678	1771	389	174	134	26,061
E47_S72	Midbrain	NLGN4	Male	8124	596	559	64	55	35	9433

Summary of signal and cell counts from the padlock probing in situ experiments. The table shows the total number of signals for each gene, and the total number of cells identified by CellProfiler, for each sample hybridized simultaneously with padlock probes for two X and Y genes