Table 3.

Distribution of cells with simultaneous detection of PCDH11X/Y and NLGN4X/Y transcripts

Sample ID	Tissue	Gene	Gender	Total cell counts	Cells with PX > = 1	Cells with PY > = 1	Cells with NX > = 1	Cells with NY > = 1	Cells with PX + Y > = 2	Cells with NX + Y > = 2	Cells with PX + NX > = 2	Cells with PY + NY > = 2
E49_S82	SC	Dual	Female	5097	247	16	282	6	0	1	38	2
E33_S63	MO	Dual	Female	17,390	993	36	1671	39	1	12	153	4
E34_S63	MO	Dual	Female	27,485	1292	71	2940	68	5	25	302	2
E32_S84	SC	Dual	Male	4939	208	241	278	353	32	49	31	16
E41_S58	MO	Dual	Male	7386	318	75	566	573	8	367	39	15
E58_S82	MO	Dual	Male	11,331	887	241	1108	977	100	361	207	50
E61_S72	MO	Dual	Male	7472	322	155	245	628	13	60	31	27
E45_S329	Midbrain	Dual	Male	26,061	1084	447	1625	1714	46	335	142	42
E47_S72	Midbrain	Dual	Male	9433	421	107	591	530	15	56	42	16
Sample ID	Tissue	Gene	Gender		Cells with PX + NY > = 2	Cells with PY + NX > = 2	Cells with PX + NX + NY > = 3	Cells with PY + NX + NY > = 3	Cells with PX + PY + NX > = 3	Cells with PX + PY + NY > = 3	Cells with PXandY + NXandY > = 4	Cells with no signals
E49_S82	SC	Dual	Female		1	0	0	0	0	0	0	4504
E33_S63	MO	Dual	Female		7	5	2	0	2	0	0	14,467
E34_S63	MO	Dual	Female		5	9	4	3	4	3	1	22,751
E32_S84	SC	Dual	Male		38	23	11	7	11	7	2	3639
E41_S58	MO	Dual	Male		43	5	50	4	50	4	0	5318
E58_S82	MO	Dual	Male		169	58	100	22	100	22	8	6991
E61_S72	MO	Dual	Male		50	15	4	3	4	3	1	5915
E45_S329	Midbrain	Dual	Male		141	74	29	20	29	20	5	20,338
E47_S72	Midbrain	Dual	Male		46	17	6	2	6	2	0	7581

The table shows the total number of signals for each gene, and the total number of cells identified by CellProfiler, for each sample hybridized simultaneously with padlock probes for the four X or Y genes: PCDH11X (PX), PCDH11Y (PY), NLGN4X (NX) and NLGNY (NY)