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. 2016 Jan 12;5:105. doi: 10.3389/fcimb.2015.00105

Figure 4.

Figure 4

Direct suppression of bacterial growth in the MLN by poly I:C requires NK cells. (A,B) In vitro Y. enterocolitica killing assay using WT or TLR4−∕− MLN cells in the presence or absence of poly I:C (n = 10 each). (C) ELISA analysis of IFN-γ production from MLN cells isolated from mice with or without poly I:C treatment (n = 4 mice each). (D) Real time PCR analysis of IFN-β, IL-12, and IL-18 expression from different innate immune cells of the MLN in response to poly I:C (n = 4 mice each, data from triplicated samples). (E) In vitro infection of MLN cells with Y. enterocolitica in the presence or absence of poly I:C together with neutralizing antibodies against IL-12, IFNAR, and IFN-γ (n = 6 each). (F) In vitro Y. enterocolitica killing assay in WT MLN cells that were depleted of NK cells. The effect of poly I:C in the presence or absence of α-IFNAR antibody (n = 10 each). *P < 0.05; NS, not significant. Error bars on graphs represent mean ± s.e.m.