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Glucose deprivation initiates apoptosis in MDA-MB-231 breast cancer cells. (A) Representative images of MDA-MB-231 reporter cells assayed in full media or media deprived of glucose (Gluc) and/or glutamine (Gln). (B) Graph shows mean values + SEMs (n = 4 per condition) of the percentages of pixels corresponding to a longer lifetime of LSS-mOrange after cleavage at the caspase-3 site (*P < .05; **P < .01).
