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. 2015 Jul 22;93(3):60. doi: 10.1095/biolreprod.115.128645

FIG. 1.

FIG. 1

Representative single cell tracings from UV endo. Representative tracings of DAF-2 and Fura-2 output recorded from cells located in the intact endothelium of umbilical vein segments (UV endo) are shown. A representative recording from control subject is shown in A. Note that UV endo was treated with the physiologic agonist ATP (100 μM) at the time shown by the arrow (time 0). The comparable data are also shown for a vessel segment from a GDM subject (B) or a DM subject (C). As a control to bypass the receptor signaling apparatus and indicate total functional NOS3 in each cell, Ca2+ ionophore (ionomycin 5 μM) was also added as indicated at time 0. D) The ionomycin response of a vessel segment from control subjects. Recording in all cases was continued for 30 min.