FIG. 5.

NOS3, GJA1 and HSP 90 expression levels in UV endo cells. Western blot analyses for NOS3 as well as for GJA1 gap junction protein (Cx43) and a control marker heat shock protein 90 (HSP90AA1) for n = 3–6 subjects per group. Western blot data for control, DM and GDM subjects demonstrate a normal range of expression, depicted by the distribution of the control data. While scatter of the data is large, and thus statistical analysis of this limited data set has little value, it is clear that immunodetectable NOS3 levels for DM and GDM subjects were not substantially reduced (differences between groups not significant).