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The effects of E2, ERα agonist PPT, and ERβ agonist DPN on cell death. Receptor-subtype-specific ligands were used to determine which subtype mediated apoptosis in HTR-8/SVneo cells. A) The effect of E2 on TUNEL indices. B) The effect of ERα agonist PPT on TUNEL indices. C) The effect of ERβ agonist DPN on TUNEL indices. Statistical differences compared to the vehicle are denoted by the asterisks (**P < 0.01, ***P < 001). Bars represent mean ± SEM (n = 3).
