Figure 5.

XCR1⁺ DCs are required for the clustering of mCTLs around the marginal zone, and promote their subsequent migration into the T cell zone. Memory Karma mice were generated as described in Fig. 3. (A) Spleen sections from untreated or DT-treated memory mice were stained with anti-B220 (dark blue) and anti-GFP (green) to define B cell follicles and OT-I mCTLs, respectively, and analyzed by confocal microscopy. Bars, 200 µm. Dotted lines delineate T cell zones. One representative experiment of three, each with three mice per group, is shown. (B) Statistical analysis of the frequency of OT-I mCTLs found clustered around the follicles at 6 h. Graph shows pooled data (mean ± SEM) from three independent experiments each with three mice per group. **, P < 0.01. (C) Statistical analysis of the frequency of OT-I mCTLs present in the T cell zones at 24 h. Graph shows pooled data (mean ± SEM) from two independent experiments each with three mice per group. **, P < 0.01; ns, nonsignificant.