Figure 4.

Batf3^−/− mice display stronger type 2 immunity to H. polygyrus infection. mLN cell numbers (A) or frequencies (B) of CD3⁺CD4⁺ T cells, CD3⁺CD8α⁺ T cells, CD19⁺ B cells, and CD3⁻DX5⁺ NK cells in mLNs from WT or Batf3^−/− BALB/c naive mice or mice infected for 11 d with H. polygyrus (100 L3 stage larvae). (C and D) mLN cells from WT or Batf3^−/− BALB/c naive mice or mice infected for 11 d as in A were restimulated with PMA/Ionomycin in the presence of Brefeldin A, after which CD4⁺ T cells were stained intracellularly for indicated cytokines. (E) mLN cells from 15 d H. polygyrus–infected mice were restimulated with H. polygyrus–derived worm antigen for 3 d and cytokine levels in culture supernatants were determined. (F) Foxp3 staining in T cells isolated from mLNs from mice as described in A. (G) Frequencies of DC subsets within the CD11c⁺MHCII^hi migratory DC (mDC) population and CD11c^hiMHCII⁺ resident DC (rDC) population in mLN from naive and 11 d H. polygyrus–infected WT or Batf3^−/− BALB/c mice. The gating strategy for mDC and rDC subsets is illustrated in the top panel, showing mLN cells from a naive WT mouse and from which CD19⁺ B cells, which can also be CD11c⁺MHCII⁺, have been gated out. Data are (C, F, and G) concatenated plots or (A, B, D, and E) shown as bar graphs representing mean ± SEM from three to six mice per group. One of two (B, F, and G) or three (A and C–E) experiments is shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001.