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. 2015 Nov 5;26(22):4015–4032. doi: 10.1091/mbc.E14-12-1632

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© 2015 Au, Hermo, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — TG fractions correspond to the germ cell Golgi apparatus with GL54D an exclusive marker for the germ cell Golgi apparatus. (A and B) EM of isolated TG fraction (A) and step 12 spermatid Golgi (B) show similar features. (C) Tomography of Golgi stacks (S) of TG fraction. (D) Heat map of 19 proteins of unknown category sorted by abundance in TG fractions. (E) Primary sequence of rat GL54D with deduced peptide sequences represented (heat map with scale bar). Monospecific polyclonal antibodies were raised to a synthetic peptide from aa 355–372. (F) Western blots of GL54D. Left, TG fraction (TG); center, aqueous extract after Triton X-114 phase partitioning; right, detergent phase. Digestions with no enzyme (Mock), PNGase F, EndoH, and Neur + O-Glyco. Asterisk indicates GL54D; arrow indicates 12-kDa shift. (G and H) IHC and (I) IF of GL54D in germ cell Golgi apparatus of spermatocytes and spermatids. Arrows indicate fragmenting Golgi apparatus of late spermatids. (J) Golgi bodies (boxes) retain Golgi identity with GL54D immunoreactivity during meiotic divisions of spermatocytes.