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. 2015 Nov 5;26(22):4015–4032. doi: 10.1091/mbc.E14-12-1632

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© 2015 Au, Hermo, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Expression alone targets GL54D to the Golgi apparatus in HepG2 cells. (A) Confocal IF of GL54D-GFP stably transfected HepG2 cells, (B) after immunostaining with monoclonal anti-galactosyltransferase and (C) colocalized (merged). (D–G) Comparison of GL54D-GFP (D) with monoclonal anti-ERMAN1 (E) and polyclonal anti-TMED7/p27 (F) and colocalized (G, merged). Confocal merge of GL54D and anti-galactosyl transferase after brefeldin A (BFA) for (H) 15 min or (I) 30 min.