Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Nov 5;26(22):4015–4032. doi: 10.1091/mbc.E14-12-1632

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Au, Hermo, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

PMC Copyright notice

FIGURE 9: — GL54D, TM9SF3, and TMED7/p27 are Golgi restricted, while MANIIX and GRASP55 access the acrosome during germ cell differentiation. (A) GL54D is restricted to the Golgi apparatus (arrowhead) adjacent to the nucleus (n) and apposed to an unreactive acrosome (horizontal arrow). Late spermatids (asterisk) show no immunoreactivity, while the Golgi of pachytene spermatocytes is immunoreactive. (B) TM9SF3 reactive in the Golgi (arrowhead) apposed to an unreactive acrosome. Golgi immunoreactivity in pachytene spermatocytes and Sertoli cells is seen while late spermatids (asterisk) are nonreactive. (C) TMED7/p27 IHC shows reactive Golgi apparatus (boxes in C) without adjacent acrosome immunoreactivity. Golgi localization to pachytene spermatocytes and forming Hermes bodies of step 19 spermatids is seen. (D) IF of MANIIX in acrosome (box) and Golgi apparatus. (E) IHC of step 9 spermatids reactive for TMED7/p27 in the Golgi during its migration. Residual bodies are immunoreactive in step 19 spermatids. (F) GRASP55 showing acrosome but not Golgi immunoreactivity (asterisk over arrowhead) after Golgi migration. (G) Schematic representation of stacks (S) of the Golgi apparatus of the step 7 spermatid overlying the nucleus (N). Proteins localized to both the Golgi and acrosome (red bracket) are indicated. Proteins restricted to Golgi apparatus (green bracket) are shown. The trans-Golgi network (TGN), cis-Golgi element (CE), and wells (W) of spermatid Golgi apparatus are indicated. Modified from Clermont et al. (1995).