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. 2015 Nov 5;26(22):4057–4062. doi: 10.1091/mbc.E15-06-0448

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© 2015 Smith et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Simulation object and PSF examples. (a) Superresolution reconstruction of a Siemens star as used in the simulations. Time projections in which each single molecule is replaced by its localization precision. (b) Zoom-in on a, visualizing the achieved resolution at the highest-frequency region of the object. (c) Examples of simulated single-molecule images as used in simulations with varying signal (I_max) and background rates (in photons) as indicated. An area of 12 × 12 pixels with σ_PSF = 1.39 pixels. (d) Single-molecule intensity distribution resulting from a Markov process simulation for the STORM experiment, where the maximum “on” time is >1 frame.