Figure 1.

Hydrolysis of Tc24 by IgM purified from unexposed mice and humans. IgM was pooled from the sera of naive Balb/c mice and from the sera of five individuals that tested negative for Chagas and Tc24 hydrolysis was assessed over a period of 48 hours. (A) Dose-dependent hydrolysis of Tc24 by mouse or human IgM (0, 25, 50, and 100 μg/mL). (B) Representative silver-stained gel demonstrating hydrolysis of Tc24 (2.6 μM) by either human or mouse IgM (100 μg/mL). Parent band depletion was measured using ImageJ.