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Specificity of IgM-mediated hydrolysis. IgM (45 μg/mL) purified from alum-only injected mouse serum was incubated with 2.6 μM of either Tc24, Na-ASP-2, Panton–Valentine bicomponent pore-forming leukotoxin (LukS-PV), toxic shock syndrome toxin (TSST), or dispersin for 24 hours at 37°C with shaking. Samples were then subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis using 15% Tris-Gly gels and silver-stained.
