Figure 6.

Temporal effects of antigen exposure on catalytic activity. Mice were infected with Trypanosoma cruzi by injection of 500 blood form trypomastigotes of T. cruzi H1 and individual mice were killed at 4, 7, 21, or 28 days postinfection. IgM (65 μg/mL) purified from individual mice killed at each time point was incubated with 2.6 μM Tc24 for 72 hours at 37°C with shaking and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis using 15% Tris-Gly gels. Percent cleavage calculated using ImageJ. IgM recovery from individual mice was determined using Micro BCA kit.