Indirect immunofluorescence assay detection of Borrelia crocidurae Achema strain (A and B) and clinical isolate B. crocidurae 03-02 (C and D) by purified mouse monoclonal antibodies (MAbs). P3A10 (panel A and C at 1:500 and 1:1,000 dilution, respectively) and P6D9 (panel B and D at 1:500 and 1:1,000 dilution, respectively). MAbs were tagged using a goat anti-mouse IgG conjugated to fluorescein isothiocyanate at 1:400 dilution. Magnification: ×1,000.