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. Author manuscript; available in PMC: 2016 Jan 12.
Published in final edited form as: Biochemistry. 2012 Aug 20;51(35):7000–7016. doi: 10.1021/bi301059m

Figure 4.

Figure 4

GC-MS spectra of 4-HB. The X and Y axes designate m/z units and relative intensity (%), respectively. (A) The 4-HB standard (parent ion m/z = 138). (B) The 4-HB produced by the (2.0 mM) wild-type thioesterase-catalyzed hydrolysis of 4-HB-CoA (0.2 mM) in natural isotopic abundance water. (C) The 4-HB produced by (2.0 mM) wild-type thioesterase-catalyzed hydrolysis of 4-HB-CoA (0.2 mM) in 97% H218O. (D) The 4-HB produced by (2.0 mM) E73D thioesterase-catalyzed hydrolysis of 4-HB-CoA (0.2 mM) in 97% H218O. (E) The 4-HB produced by (2.0 mM) T77A thioesterase-catalyzed 4-HB-CoA (0.2 mM) hydrolysis in 97% H218O. (F) The 4-HB produced by (0.02 mM) T77A thioesterase-catalyzed hydrolysis of 4-HB-CoA (0.2 mM) in 97% H218O. (G) The 4-HB produced by the (2.0 mM) T77S thioesterase-catalyzed hydrolysis of 4-HB-CoA (0.2 mM) in 97% H218O. (H) The 4-HB produced by (2.0 mM) E73D/T77A thioesterase-catalyzed hydrolysis of 4-HB-CoA (0.2 mM) in 97% H218O.