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CoA and 4-HB steady-state kinetic inhibition constants measured for wild-type and mutant thioesterases at pH 7.5 and 25 °C. See Materials and Methods for details.
| Thioesterase | CoA | 4-HB | |
|---|---|---|---|
| Kis (μM) | Kii (μM) | Kis (μM) | |
| Wild-type | 16 ± 2 | 29 ± 2 | 240 ± 80 |
| E73D | 32 ± 2 | -------- | 1700 ± 400 |
| S120A | 110 ± 30 | 110 ± 10 | -------- |
| T121A | 360 ± 50 | 580 ± 60 | -------- |
| R150A | 460 ± 80 | 700 ± 700 | -------- |
| R102A | 500 ± 100 | 1200 ± 300 | -------- |
