Fig. 4.

Whole-mount immunofluorescence staining of the 4 dpf zebrafish larvae (a), 22 dpf juvenile zebrafish (b) using antibody against galanin and adrenergic marker—tyrosine hydroxylase (TH). Arrow points to galaninergic neurons in autonomic ganglia. Arrowheads show the primary islet of the endocrine pancreas. Galanin-IR cells and fibers did not express immunoreactivity to TH. However, very intensely stained TH-IR neurons were found in the celiac ganglion (a) and in the brain (b). CeG celiac ganglion, E eye, I intestine, Me mesencephalon, Rho rhombencephalon. Scale bars 100 µm