Table 1.
Primary and secondary antibodies used in the study
| Antigen | Immunogen | Host | Clonality | Dilution | Company | Catalog no. |
|---|---|---|---|---|---|---|
| Primary antibodies | ||||||
| Galanin | Rat galanin synthetic peptide | Rabbit | Polyclonal | 1:5000 | Millipore | AB2233 |
| HuC/D | Human HuC/D neuronal protein | Mouse | Monoclonal | 1:1000 | Invitrogen | A-21271 |
| AcTub | Acetylated alpha-tubulin from the axoneme of sea urchin sperm flagella | Mouse | Monoclonal | 1:1000 | Invitrogen | 32-2700 |
| TH1 | Tyrosine hydroxylase purified from PC12 cells | Mouse | Monoclonal | 1:2000 | Millipore | MAB318 |
| ChAT | Human placental enzyme | Goat | Polyclonal | 1:100 | Millipore | AB144P |
| Sst | Somatostatin conjugated to thyroglobulin | Rat | Monoclonal | 1:100 | Serotec | 8330-0009 |
| Antigen | Fluorophore | Host | Dilution | Company | Catalog no. |
|---|---|---|---|---|---|
| Secondary antibodies | |||||
| Rabbit IgG | Alexa 635 | Goat | 1:1000 | Invitrogen | A-31576 |
| Mouse IgG | Alexa 488 | Goat | 1:1000 | Invitrogen | A-11029 |
| Rabbit IgG | Alexa 555 | Goat | 1:1000 | Invitrogen | A-21431 |
| Rabbit IgG | Alexa 488 | Donkey | 1:1000 | Invitrogen | A-21206 |
| Goat IgG | Alexa 555 | Donkey | 1:1000 | Invitrogen | A-21432 |
| Rat IgG | Alexa 488 | Goat | 1:1000 | Invitrogen | A-11006 |