Table 3.
Selection steps applied to highlight H. incana candidate genes regulated in response to Pb exposure in roots and shoots.
| Category | Treatment | Roots | Shoots | ||
|---|---|---|---|---|---|
| Specific genes | Total | 341 | 601 | ||
| FC > 2.0 or FC < 0.5 | 62 Up-reg | 59 Down-reg | 192 Up-reg | 138 Down-reg | |
| Common genes | Total | 1102 | 63 | ||
| FC Hi/FC At > 2.0 or < 0.5 | 315 | 28 | |||
| FC > 2.0 or FC < 0.5 | 125 Up-reg | 102 Down-reg | 18 Up-reg | 8 Down-reg | |
| Candidate genes | 187 Up-reg | 161 Down-reg | 210 Up-reg | 146 Down-reg | |
H. incana specific genes were directly chosen on the basis of their fold change (FC) ratio (FC > 2.0 or FC < 0.5). H. incana common genes were first selected on the basis of their FC related to those from A. thaliana (FC Hi/FC At > 2.0 or < 0.5) and then FC on the basis of the FC ratio (FC > 2.0 or FC < 0.5). (Fold Change, FC; Hi, H. incana; At, A. thaliana; down-reg, down-regulated genes; up-reg, up-regulated genes).