Figure 2.

Profiling EB volume and cellular density. (A) CLSM z-axis stacks were collected through the entire depth of PI-stained EBs and were reconstructed in maximum projection images (Max). Sections a, b, and c correspond to sections taken at 75% (a), 50% (b), and 25% (c) of the depth of the EB, where 0% is the EB-glass interface. The entire z-axis image sequences were reconstructed for EBs at 0, 1, 2, and 3 weeks and are given in Supplementary Movies 2–5, respectively. (B) EB volumes are given as mean ± SE (n = 3–6 stacks from separate EBs). (C) The proportion of EB volume calculated in (B) which was occupied by PI-stained cells was expressed as a percentage. Data are given as mean ± SE (n = 3–6 stacks from separate EBs).