Figure 8.

Effect of increased [Ca²⁺]_ext in quiescent IPS-CMs. (A) Color-coded visualization of the proportion of IPS-CMs that exhibited spontaneous Ca²⁺ oscillation following their disaggregation from EBs at different time periods (horizontal) or their transfer to “on plate” culture (vertical). (B,C) The proportion of IPS-CM across the experimental matrix that responded to increased [Ca²⁺]_ext by a sustained non-oscillatory elevation in [Ca²⁺]_cyt [typical of that shown in (B)] is visualized in (C). (D,E) The proportion of IPS-CM across the experimental matrix that responded to increased [Ca²⁺]_ext by sustained [Ca²⁺]_cyt oscillations around an elevated [Ca²⁺]_cyt [typical of the pattern shown in (D)] is visualized in (E). In (C,E) the mean data of n = 3 plates at each time point are given.