FIGURE 3.

The promotion of endocytic vesicle recycling during treatment with UV-B irradiance (0.3 mW/cm²) for 20 min and Brefeldin-A (BFA). (A) Representative fluorescence images of UV-B-induced formation of BFA-compartments in root tip epidermal cells. Red spots represent BFA-compartments. Plasma membranes were stained with FM4-64 fluorescent dye. BFA concentration was 35 μM. (B) The effect of background illumination emitted by the UV-B lamp covered with a polyester film. Scale bars indicate 20 μm. (C) Comparison of the size of BFA-induced compartments in root epidermal cells. Roots were irradiated for 20 min with UV-B with or without polyester film. BFA compartments (33 in control, 104 in UV-B, 69 in UV-B with cutoff filter from 3 to 10 independent treated roots) were counted and their diameters were averaged. Different letters on the bars indicate significant difference tested by Tukey’s HSD test, p < 0.05.