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. 2015 Aug 11;77(12):1617–1624. doi: 10.1292/jvms.15-0289

Table 1. Summary of miRNA primers used in real-time RT-PCR.

Name Sequence (5′→3′) Length (nt) GC (%) Tm
U6 AGGATGTGAAGACACCAAGACA 22 45.45 59.62
oar_miR_3958_3p AGATATTGCACGGTTGATCTCT 22 40.9 55.9
oar_miR_432 TCTTGGAGTAGGTCATTGGGTGG 23 52.2 62.8
oar_miR_379_5p TGGTAGACTATGGAACGTAGGC 22 50.0 56.6
oar_miR_485_5p AGAGGCTGGCCGTGATGAAT 20 55.0 61.8
oar_miR_127 ATCGGATCCGTCTGAGCTTG 20 55.0 59.9
ovis_aries_ovary-m0005_5p GCGAAAGGTTCATTTGGGTT 20 45.0 59.2
ovis_aries_ovary-m0032_5p CAGGCTAGGAGAAATGATTGG 21 47.6 56.8
ovis_aries_ovary-m0033_3p ACAGCAGGCACAGACAGGC 19 61.1 58.4
ovis_aries_ovary-m0070_5p GCTGGAAGACTAGTGATTTTGTTG 24 41.7 58.1
ovis_aries_ovary-m0083_3p TCACAGTGAACCGGTCTCTTT 21 47.6 57.0
ovis_aries_ovary-m0088_5p CAACGGAATCCCAAAAGCAGC 211 52.4 64.4
ovis_aries_ovary-m0098_3p TTCACCACCTTCTCCACCC 19 52.9 52.1
ovis_aries_ovary-m0105_5p GCAAAGAATTCTCCTTTTGGGC 22 45.5 62.2
ovis_aries_ovary-m0132_5p AGGCGGAGACTTGGGCAATT 20 55.0 63.4
ovis_aries_ovary-m0161_3p TCACAGTGAACCGGTCTCTTT 21 47.6 57.0