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. 2016 Jan 13;6:1527. doi: 10.3389/fmicb.2015.01527

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Copyright © 2016 Estrada-Mata, Navarro-Arias, Pérez-García, Mellado-Mojica, López, Csonka, Gacser and Mora-Montes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of β1,3-glucan and chitin exposure at the cell wall surface. Live (L) yeast cells were incubated with IgG Fc-Dectin-1and then with FITC-conjugated IgG to label β1,3-glucan (closed circles). Alternatively, chitin was labeled with WGA-FITC (open circles). The fluorescence associated to each cell is plotted. In addition, the experiment was conducted with cells previously heat-inactivated (HK). Strains used are Candida parapsilosis sensu stricto SZMC 8110, C. orthopsilosis SZMC 1545, C. metapsilosis SZMC 1548, and C. albicans SC5314. One hundred cells were analyzed for each group. ‡P < 0.05, when compared with the live cells; †P < 0.05 when compared to the other groups with live cells.