Figure 1.
The Ntsr-1 cre drives expression of tdTomato in layer VI projection neurons to the thalamic reticular nucleus and ventrobasal thalamus. A, Stitched 10× images of the projections from layer VI through the internal capsule to the nRT and thalamus. Cell nuclei are marked with DAPI in blue, TdTomato staining in red indicates Ntsr Cre+ cells, and FITC staining in green marks parvalbumin interneurons throughout the brain. Scale bar, 500 μm. B, Image (10×) of layer VI cortical projection neurons. Scale bar, 200 μm. C, Image (10×) of the nRT and thalamus shows no cre-driven cellular staining, but diffuse label due to axonal projections from layer VI neurons. Scale bar, 200 μm. D, Image (10×) of hippocampal dentate gyrus shows scattered cells expressing tdTomato. Scale bar, 200 μm.