Figure 2.

Functional effects of 4-PIOL on steady-state GABA currents. Representative whole-cell currents induced by long applications of 0.1 and 1 μm GABA to α4β2δ (A), α6β2δ (B), α5β3γ2 (C), and α1β3γ2 (D) receptors. 4-PIOL (10 μm) was briefly coapplied once a steady-state GABA current was achieved. E, Quantitative analysis of data depicted in A–D. Data points for α1β3γ2 (♦), α5β3γ2 (■), α6β2δ (▴), and α4β2δ (●) receptors are mean ± SEM (n = 4–5 cells). For each concentration (0.1, 0.3, and 1 μm) of preapplied GABA, the change in holding current produced by 4-PIOL is expressed as a percentage of the steady-state current to GABA alone. Negative values represent an inhibition of the steady-state GABA current.