Table 5.

Comparison between heart failure and hypothyroid energy metabolism.

		Sham rats$	CHF rats$	CT	HYPO
Mitochondrial respiration	Vmax (μmol O2.min−1.g−1dw)	26.4 ± 1.5	20.9 ± 1.0 **	24.9 ± 0.9	19.3 ± 0.7 **
Mitochondrial enzyme activities (IU/g prot)	CS activity	603 ± 18	378 ± 23 ***	933 ± 48	777 ± 33 *
	COX activity	1516 ± 69	962 ± 82 ***	1787 ± 165	1306 ± 130 *
Mitochondrial biogenesis : mRNA content (arbitrary unit)	PGC-1a	2.69 ± 0.26	1.82 ± 0.14 **	0.8 ± 0.07	0.69 ± 0.04
	NRF1	0.77 ± 0.07	0.72 ± 0.04	0.25 ± 0.02	0.25 ± 0.03
	NRF2	1.28 ± 0.11	0.87 ± 0.08 **	0.2 ± 0.02	0.2 ± 0.02
	Tfam	0.61 ± 0.04	0.41 ± 0.02	0.29 ± 0.03	0.27 ± 0.03
Energy transfer (IU/g prot)	Total CK activity	4276 ± 80	1966 ± 125 ***	3742 ± 230	3367 ± 130
	M-CK activity	2021 ± 137	1210 ± 61 **	2612 ± 227	2600 ± 170
	mi-CK activity	1303 ± 101	201 ± 38 ***	743 ± 63	604 ± 42
	AK activity	1286 ± 202	978 ± 88	1209 ± 93	1477 ± 82
Substrate utilization : enzyme activities (IU/g prot)	H-LDH	2072 ± 76	1478 ± 63 ***	1487 ± 51	1499 ± 59
	M-LDH	883 ± 46	839 ± 56	934 ± 34	828 ± 36 *
	H-LDH/M-LDH	2.35 ± 0.07	1.76 ± 0.12 **	1.59 ± 0.03	1.83 ± 0.1

^$All values on sham and CHF rats come from Garnier et al J Physiol 2003, except for the energy transfer and the substrate utilization data, which come from De Sousa et al Circ Res 1999. Sham and CHF (congestive heart failure) rats data, originally expressed by g wet weight, were converted in mg of protein using a protein to wet weight ratio of 18%.

^*p<0.05,

^**p<0.01,

^***p<0.001 between sham and CHF rats, or between Eu and Hypothyroid rats. The mRNA content of Sham and CHF rats were normalized to β-actin transcription, while Eu and Hypothyroid rats mRNA are normalized to cyclophylin A.