Fig. 4.

TEER and the percentage of blood monocytes that migrated across the BBB in different models. The cell-induced as well as co-culture TEER values were evaluated by subtracting the TEER of the blank insert. (A) Percentage of spontaneous migration vs. TEER values. (B) Percentage of specific migration toward CCL19 vs. TEER values. For the last condition, monocytes were treated for 24 h with PGE2 before the migration assay through the BBB model. Data represent the mean of four independent experiments, ±SD. Statistical analysis was performed using Student t-test; *p < 0.05; **p < 0.01, ***p < 0.001, ****p < 0.0001).