Figure 2.

Mitochondrial dynamics during cell division. A-D. Doubly transgenic lines expressing unCPS-YFP and CFP-H4. Green, blue and red fluorescence correspond to mitochondrion, nucleus and chloroplast, respectively. E and G. Mitochondrion and nucleus stained with MitoTracker Orange and DAPI. F and H. DIC images of same cells in E and G respectively. A. Branched mitochondrion is stretched to the tip of the cell along the chloroplast. B. Two spots of mitochondrial signals beside the chloroplast constrictions (arrowheads). C. Extended mitochondria located in the future dividing plane prior to karyokinesis. D. Two identical cells with elongated mitochondria just after cell division. E. Branched mitochondrion is extended throughout the cell. DIC image of the cell is shown in F. G. Signals from mitochondria accumulate between two daughter nuclei (arrowheads). DIC image is shown in H. Scale bar: 1 μm.