Figure 3.

Actin dynamics throughout the cell cycle. A-G. F-actin, nucleus and chloroplast were visualized with FITC-phalloidin (green), DAPI (blue) and chlorophyll autofluorescence (red), respectively. A. Interphase cell surrounded by actin bundle under the plasma membrane. B. Actin ring appeared after the duplication of chloroplast. C. Contracted actin ring with fibrous actin in the cell. D. Smaller contractile ring after karyokinesis. The ring positioned between two daughter nuclei. E. Oblique view of the cell at a similar stage as in D. Actin fibers connected with the contractile ring. F. Final stage of cytokinesis. Actin accumulated in the cytokinetic face. G. Completion of cell division. Two daughter cells were surrounded by actin bundles. H. Bright field image in same frame with G. Cell walls for both daughter cells had been completely generated. Scale bar: 1 μm.