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. 2016 Jan 13;8:89. doi: 10.3389/fnmol.2015.00089

Figure 4.

Figure 4

MEK inhibition abolishes morphological effect of Negr1. Cortical neurons were infected with siControl virus at DIV4 and treated every second day from DIV10 to DIV18 with DMSO or with MEK inhibitor U0126 (100 nM, IN) and/or recombinant Negr1 (40 ng/ml, single administration at DIV 10, rNegr1). Neurons were processed for immunofluorescence at DIV18 and infected GFP positive neurons imaged via confocal microscopy. Panels show camera lucida tracing (A). Graphs show neurite total length and number (B). Data are reported as mean ± SEM; *p < 0.001 vs. not treated (NT), °p < 0.001 vs. rNegr1. Scale bar = 50 μm.