Table 1.
MALDI-IMS summary effects of NNK, ethanol, and NNK+ethanol exposures on phospholipid (PI, PS) and sphingolipid (ST) ion intensities (abundances) in rat brains relative to control
| m/z | Lipid assignment | NNK | Ethanol | NNK/Ethanol |
|---|---|---|---|---|
| 705.7 | PS(30:0) | ↔ | ↔ | ↑ |
| 788.8 | PS(36:1) | ↑↑ | ↑ | ↓ |
| 834.8 | PS(40:6) | ↔ | ↔ | ↓ |
| 806.9 | ST(18:0) | ↓ | ↓↓ | ↓↓↓ |
| 888.7 | ST(24:1) | ↔ | ↓ | ↓↓ |
| 890.7 | ST(24:0) | ↓ | ↓ | ↓↓ |
| 904.8 | ST(24:1)(OH) | ↑ | ↓ | ↓↓ |
| 906.9 | ST(24:0)(OH) | ↓ | ↓↓ | ↓↓↓ |
| 857.6 | PI(36:4) | ↔ | ↓ | ↓↓ |
| 862.7 | PI(36:1) | ↓ | ↓ | ↓↓ |
| 883.8 | PI(38:5) | ↔ | ↔ | ↓ |
| 885.5 | PI(38:4) | ↔ | ↓ | ↓↓ |
| 886.8 | PI(38:3) | ↔ | ↓ | ↓↓ |
Tandem mass spectrometry (MS/MS) with MALDI LIFT-TOF/TOF was used to fragment phospholipids and sphingolipids in negative ion mode. The product ion spectra were searched against Lipid Maps database. Differential lipid intensities among groups were determined based on mass spectrum peak intensities and ion distribution map in pseudo-color representing the relative ion intensity.