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. 2015 Dec 10;18(1):51–64. doi: 10.1021/acscombsci.5b00126

Figure 2.

Figure 2

Scaffold ranking of libraries 531, 914, 1275, 1952, 1953, 1954, 1481, 1433, 1989, and 2048. (A) Screening by in vivo of antinociception response in the mouse 55 °C warm water tail-withdrawal test. All mixture-based samples were administered at a dose of 5 mg/kg, i.p. (green bars) and 25 mg/kg i.p. (red bars). For structures of the libraries see Figure 1. Response to vehicle (10% DMSO, i.p.) represented as dashed line; dotted lines represent SEM. Data represent average (±SEM) summed tail-withdrawal latencies calculated by taking the sum of the average tail withdrawal latencies for each animal from each time point over a 24-h period. Bars =7–8 mice each. B: Screening by respiration response. All mixture-based samples were administered at a dose of 5 mg/kg, i.p.; vehicle (10% DMSO, i.p.) was administered as a control. Data represent average (±SEM) respiration rate (in breaths per minute, BPM) over a 90 min period. Bars =8 mice each, except vehicle, which was 40 mice. *Significantly different from response to vehicle, p < 0.05, one-way ANOVA followed by Dunnett’s post hoc test.