Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Jan 13;16:15. doi: 10.1186/s12870-016-0705-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Lee et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 5 — Western blot analysis for MtRDR1 in RDR1-enriched protein extracts. Leaves of empty vector control transgenic or MtRDR1-transgenic N. benthamiana plants were infiltrated with a control solution of water containing 0.05 % (v/v) ethanol (-) or 2.5 mM SA (+). RDR1-enriched protein extracts were prepared 48 h later and subjected to western blot analysis with either preimmune rabbit serum (left panel) or polyclonal rabbit anti-MtRDR1 serum (right panel). The positions of protein molecular mass markers are indicated (kDa). A protein (indicated with a star) corresponding to the predicted size of 131 kDa for MtRDR1 was specifically detected in SA-treated MtRDR1-transgenic plants with the anti-MtRDR1 serum but not with the preimmune serum. Equal loading of gel lanes with protein, based on the Bradford [59] assay was confirmed by Ponceau S staining of the western blot membrane (lower panels)