Fig. 2.

Serum of a patient with acute respiratory distress syndrome (ARDS) induced stiffening on primary monocytes and neutrophils. Entry times (ETs) measured for THP-1 cells (open bar), primary monocytes (black bars), and primary neutrophils (gray bars) after 1-h incubation with the sera of a healthy donor and of a patient with ARDS. The number of tested cells per condition was >30. Values are mean ± standard deviation. *p < 0.05; ***p < 0.0001 (Mann-Whitney U test). For neutrophils incubated in serum of patients with ARDS, cells never completed their entry in C2 at 300 seconds and experiments were stopped. ETs are reported as >300 seconds, and there is no error bar