Fig. 8.

Demonstration of image analysis using Aperio® ImageScope™ software. A positive pixel count algorithm was used to quantify positive staining in the submucosa (blue region) of bronchial biopsies for tryptase, ECP, NE, CD138, CD4, and IL-4. The airway epithelium was not examined and positive staining in the epithelium was excluded from analysis. a Representative image of tryptase positive staining from a subject exposed to FAA, Black arrows denote positive staining in submucosa area that are selected by the positive pixel count. b Image from a with submucosa region selected by manual trace followed by positive pixel count recognition of tryptase stain (red colour) within submucosa region (blue colour)