FIG 4.

Whole-cell radioimmunoprecipitation assay using 11% acrylamide gels with serum samples from four children with acute NTHi otitis media. Each serum sample was tested against the NTHi strain isolated from the middle ear fluid specimen of that same child. Cells, radiolabeled proteins present in the labeled cells of the NTHi strain isolated from the indicated child; Ac, radiolabeled proteins immunoprecipitated by antibody present in the acute-phase serum of the indicated child; Con, radiolabeled proteins immunoprecipitated by antibody present in the convalescent-phase serum sample of the indicated child. Ordinate: molecular mass in kilodaltons.