TABLE 2.
Opsonophagocytic titers against the homologous NTHi strains of sera from four adults pre- and post-adsorption with HMW1 and HMW2 proteins or with serum albumina
| Serum prep | Titer by strain |
|||
|---|---|---|---|---|
| 5 | 12 | 16 | 17 | |
| Adult 1 | ||||
| Preadsorption | 20 | <10 | <10 | <10 |
| HMW-adsorbed | <10 | <10 | <10 | <10 |
| Alb-adsorbed | 20 | <10 | <10 | <10 |
| Adult 2 | ||||
| Preadsorption | 320 | 160 | <10 | 320 |
| HMW-adsorbed | 40 | 40 | <10 | 40 |
| Alb-adsorbed | 320 | 160 | <10 | 320 |
| Adult 3 | ||||
| Preadsorption | 10 | 80 | 1280 | 1280 |
| HMW-adsorbed | <10 | 20 | 640 | 640 |
| Alb-adsorbed | 10 | 80 | 1280 | 1280 |
| Adult 4 | ||||
| Preadsorption | <10 | <10 | 40 | <10 |
| HMW-adsorbed | <10 | <10 | <10 | <10 |
| Alb-adsorbed | <10 | <10 | 40 | <10 |
a
A single serum sample from each adult volunteer was adsorbed on each of five different affinity columns coupled with purified HMW1 and HMW2 proteins from strain 5, 12, 16, or 17, or with albumin (Alb) as a control. The preadsorbed sample, the HMW1- and HMW2 -adsorbed sample, and the albumin-adsorbed control sample were each assayed for their ability to mediate killing of the respective homologous NTHi strain.