FIG 4.

Biomass in biofilms (measured as OD₅₉₅) between L. pentosus and C. boidinii strains. Biofilm formation was assayed in a coculture method consisting of a two-compartment system in which L. pentosus strains and the yeast were separated by a membrane (for more details, refer to Materials and Methods). (A) The ordinate axis shows the absorption of crystal violet extracted from crystal violet-stained biofilms. The mean values and standard deviations are shown. (B) Picture of the lower compartments showing biofilm formation by L. pentosus and C. boidinii strains. (A and B) Lp13, L. pentosus Lp13 in monoculture; Lp209, L. pentosus Lp209 in monoculture; Lp13B4, L. pentosus Lp13B4 in monoculture; Cb, C. boidinii in monoculture; Lp13+Cb, L. pentosus Lp13 and C. boidinii in coculture; Lp209+Cb, L. pentosus Lp209 and C. boidinii in coculture; Lp13B4+Cb, L. pentosus 13B4 and C. boidinii in coculture. *, values are statistically significant (P ≤ 0.05).