FIG 3.

Effect of plasmid-based overexpression of the identified sulfur amino acid metabolic genes in WT S. griseus. (A) HPLC-fluorescence chromatograms of intracellular Cys and Hcy detected in the transformed strains. (B) Relative percentages of intracellular Cys and Hcy. For each strain that is a mixture of 3 to 5 individual colonies, the percentage is an average of three independent measures. (C) ABM production level. The introduced plasmids are described in Table 1. Each production level is the average of at least three experimental replicates; the standard error is represented by an error bar. Statistical significance was determined by comparing the level of a specific strain to the control. **, P < 0.01; NS, not significant.