Figure 1.

Respiratory syncytial virus (RSV) induced mammalian target of rapamycin (mTOR) phosphorylation on CD8 T cells. Peripheral blood mononuclear cells (PBMCs) (4 × 10⁵/well) were culture in RPMI 2% of fetal calf serum (FCS) with 10² plaque‐forming units (PFU) of RSV, phorbol myristate acetate (PMA) or control [uninfected human epithelial type 2 (Hep‐2) cells processed similarly to RSV‐infected Hep2 cells) for 15 or 30 min, then cells were fixed, permeabilized and stained with anti‐phospho mTOR Ser2448, followed by anti‐rabbit immunoglobulin (Ig)G cyanin 3 (Cy3) and analysed on a flow cytometer or the fluorescence microscope after cytospin and mTOR staining. (a) Graph showing the frequency of CD8⁺mTORser2448⁺ cells. (b) Histograms showing mTOR phosphorylation when cells were incubated with RSV compared to control and PMA. (b) Images of mTOR phosphorylation at ser2448 in BMCs incubated with RSV or control (blue = nuclei staining, red = mTORser2448 staining).