Figure 2.

Rapamycin inhibited respiratory syncytial virus (RSV) induction of mammalian target of rapamycin (mTOR) phosphorylation on CD8 T cells. (a) Frequency of CD8⁺mTORser2448⁺ cells after peripheral blood mononuclear cell (PBMC) incubation with 20 ηg/ml of rapamycin or 50 µM of phosphoinositide 3‐kinase/protein kinase B (PI3K/AKT) inhibitor (LY294002) 1 h before RSV incubation for 30 min. (b) PBMC were incubated with 10² plaque forming units (PFU), RSV or control for 30 min and stained with anti‐4EPB1. Graph shows mean fluorescence intensity (MFI) of 4BP1 on RSV‐stimulated cells comparing to control. (c) Frequency of CD8⁺mTORser2448⁺ cells after PBMC incubation with ultraviolet (UV)‐inactivated RSV, RSV and control for 30 min. (d) Frequency of CD8⁺mTORser2448⁺ T cells after incubation with 0·5 μg or 1 μg of RSV F protein and media for 30 min. One‐way analysis of variance (anova) with Bonferroni's multiple comparison post‐test (*P < 0·05 and **P < 0·01).