Fig. 12.7.

Flavivirus genome replication. (a) Minus-strand RNA synthesis. The viral RdRp uses a plus-sense viral genome as a template to synthesize minus-strand RNA without a primer (de novo). Minus-strand RNA forms a dsRNA with plus-sense RNA. (b) Plus-strand RNA synthesis. Plus-sense RNA is synthesized using a minus-strand RNA in a dsRNA intermediate as a template via de novo initiation. In flavivirus, the newly synthesized plus-strand RNA is capped at its 5′-end by the viral replication complex