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Speed comparison of several popular programs with Gctf. All parameters of each program were set as the default (CTFFIND3/4 called by Relion). Due to the large speed differences among programs, they were tested using different number of micrographs for multiple times: CTFFIND3 on one micrograph, CTFFIND4, FASTDEF, ACE2 on 10 micrographs, while Gctf on 1000 micrographs. Gctf was running on a single GTX 980 GPU and the other programs on Intel Xeon E5-2643 v2 CPU.
