Fig. 9.

CTF determination of single frames of a movie. (a)Averaged movie (top) and single frame (bottom) of dynactin (Dataset-5). Movie was taken on FEI Titan Krios, Falcon II detector at the dose of 1.6 e/(Ų·frame). (b)CTF determination using the averaged movie (top) or a single frame (bottom). For both images, the left is simulated CTF and the right is observed LAS. The determined defocus $(z_u\text{,}{z}_v\text{,}{\theta }_{\mathit{ast}})$ of the averaged movie is (41,642.58 Å, 41,140.62 Å, 61.67°) and the first frame is (41,711.86 Å, 41,196.36 Å, 52.80°). The difference is (69.28 Å, 55.74 Å, 8.87°). (c) The changes of averaged defocus $(z_u+z_v)/2$ with the accumulation of doses on the micrograph. Slightly different from (b), the CTF determination for each frame was performed by averaging 9 adjacent frames (e.g. 11–19 for frame 15) to enhance the SNR.